What types of gene regulatory dynamics do cells use?

Projects include:

Activation dynamics of bacterial alternative sigma factor circuits. Using time-lapse microscopy we have found bacterial cells can pulse on and off activation of alternative sigma factors. We have observed a range of dynamic behaviours that can be explained by simple mathematical models. See Park et al., 2018, Patange et al., 2018, Young et al., 2013, Locke et al., 2011).

Variable gene expression in Arabidopsis. We used RNA‐seq on individual seedlings  to detect inter‐individual gene expression variability in Arabidopsis thaliana. We found 9% of the transcriptome is highly variable for at least one time point during the day/night cycle.​ Highly variable genes tend to be involved in the response to the environment. They also tend to be shorter, to be targeted by a higher number of transcription factors and to be characterised by a “closed” chromatin environment. See Cortijo et al., 2018

Gene expression variability for individual genes of interest can be viewed on https://jlgroup.shinyapps.io/AraNoisy/.

©2019 by Locke Lab